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*Column headings for Microbial_physiochem.csv*
Sample ID = Identification tag for sample site on Sunday Island, Kimberley
Habitat = habitat where the sample was taken from
Characteristics = characteristics of the sample
Date = sampling month and year
Wet season = sample taken either pre or post wet season
Salinity = salinity measured in PSU
BP ug C/L/day = bacterial production measured with 3H-leucine and expressed as microgram carbon produced per L per day
Bac abundance x109/L = bacterial abundance measured using epifluorescence microscopy and SYBR gold staining and expressed
in number of bacteria x109 per L
Bac biomass ug C/L (10fgC per cell assumed) = bacterial biomass, estimated from assuming an average bacterial cell carbon
content of 10 femtogram and expressed as microgram carbon per L.
BIOLOG richness = number of substrates with a positive response, i.e. utilization by bacteria. Total number of substrates
per plate is 31.
BIOLOG Shannon-Weaver diversity = Shannon-Weaver’s diversity indices (SDIs) were calculated as follows:
SDI= −Σpi(lnpi)
where pi is the ratio of normalized optical density at 595nm on each substrate to the sum of optical density at 595nm on
all substrates.
BIOLOG NUSE (Nitrogen Use Index) = proportion of utilized substrates containing both C and N relative to total substrate utilization
on the BIOLOG plates. Gives indication of extent of microbial nitrogen usage.
Total carbohydrates ug/g = Concentration of total carbohydrates in the sample expressed in microgram carbohydrates per gram
sediment.
DOC mg/L = dissolved organic carbon in the sample, expressed as mg per L, for sediment samples measured in porewater compartment.
TDN mg/L = total dissolved nitrogen in the sample, expressed as mg per L, for sediment samples measured in porewater compartment.
DOC:TDN = dissolved organic carbon to total dissolved nitrogen ratio.
SUVA = Specific ultraviolet absorbance, calculated by dividing absorbance at 254nm by the dissolved organic carbon concentration.
For sediment samples measured in porewater compartment.
FI = Fluorescence index calculated as the ratio of emission intensity at 450 nm to that of 500 nm for a fixed excitation
wavelength of 370 nm. For sediment samples measured in porewater compartment.
% aromaticity = aromaticity of the sample, i.e. hydrophobic C content, calculated as follow: (0.05(absorbance280nm *1000))
+ 6.74. For sediment samples measured in porewater compartment.
Total chl a ug/L or ug/g = chlorophyll a in the sample expressed either as microgram per L or microgram per gram.
Total phaeophytin ug/L or ug/g = phaeophytin in the sample expressed either as microgram per L or microgram per gram.
> 5 um chl a ug/L = chlorophyll a in the fraction above 5 micron in size expressed as microgram per L.
> 5 um phaeophytin ug/L = phaeophytin in the fraction above 5 micron in size expressed as microgram per L.
NH4 umol/L = ammonia expressed as micromole per L.
PO4 umol/L= phosphate expressed as micromole per L.
Si umol/L = silicone expressed as micromole per L.
%water (moisture content for sediment)= percentage water content in the sediment.
TSS mg/L = total suspended solids expressed in milligram per L.
15N = 15N stable isotope content.
13C = 13C stable isotope content.
N mg = nitrogen content expressed in milligram
C mg = carbon content expressed in milligram
C:N = carbon to nitrogen ratio in sample.
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